 Validation of Molecular Detection Methods for Gluten Allergens in Infant FormulaZHAO，Shuhuan, WANG，Yunxia and Cuizhi Li Asian Agricultural Research, 2024, vol. 16, issue 01 Abstract: [Objectives] To verify the specificity, sensitivity, precision and negative-positive deviation of the foodproof gluten component detection kit for the detection of gluten allergens in milk powder matrix, and to establish a real-time fluorescent PCR legal method for the detection of gluten allergens in milk powder. [Methods] The specificity, sensitivity, precision and negative-positive deviation of the detection method of foodproof gluten component detection kit (PCR-probe method) were verified by artificially adding different concentrations of wheat bran and extracting sample DNA by kit method, and applied to sample detection. [Results] The specific detection results of two kinds of milk powder with wheat bran and buckwheat added showed that the foodproof gluten component detection kit (PCR-probe method) had good specificity for wheat gluten. The results of artificially added wheat bran positive samples showed that the false positive rate and false negative rate of the kit in the milk powder matrix were 0, and the sensitivity and precision were high. [Conclusions] The kit is simple to operate and has high accuracy, which is suitable for the detection of gluten allergen components in milk powder. Keywords: Agribusiness (search for similar items in EconPapers) Downloads: (external link) Related works: Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text Persistent link: https://EconPapers.repec.org/RePEc:ags:asagre:344157 Access Statistics for this article More articles in Asian Agricultural Research from USA-China Science and Culture Media Corporation |
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