Isolation and Identification of Potassium Releasing Bacteria from Sugarcane Rhizosphere Soil and Optimization of Fermentation Condition

Huanhuan Jiang, Honghuo He, Cuiting Mo, Sainan Li and Gang Chen

Asian Agricultural Research, 2024, vol. 16, issue 12

Abstract: [Objectives]To isolate and optimize the potassium releasing bacteria from sugarcane rhizosphere soil in Baitu Town, Gaoyao District, Zhaoqing City, Guangdong Province, and then evaluate their potassium-releasing ability, and optimize the fermentation conditions of the strains with the best potassium-releasing ability, so as to provide a scientific basis for the development and production of potassium releasing bacteria fertilizer. [Methods] Potassium-solubilizing bacteria were isolated from sugarcane rhizosphere soil using potassium-feldspar powder as the potassium source of isolation medium by dilution coating method and plate streaking method. The isolated strains were identified by 16s rRNA sequence analysis. The potassium-releasing ability of each strain was determined by sodium tetraphenylborate (STPB) method, and the strain GK-37 with the optimal potassium-releasing ability was selected. The fermentation conditions of GK-37 strain were further optimized, and the effects of different carbon sources on its growth were mainly investigated. [Results] Seven strains of potassium-solubilizing bacteria were isolated and purified, and identified by 16s rRNA. They belonged to Pseudomonas knackmussii, Pseudomonas, Pseudomonas insulaes and Caballeronia zhejiangensis. Among the tested strains, strain GK-37 had the best potassium-releasing ability, and its potassium-releasing capacity was 26.99 mg/L. By optimizing the fermentation conditions of GK-37 strain, it was found that when the fermentation medium was sucrose as carbon source, the growth of the strain was the best. [Conclusions] In this study, the potassium releasing bacteria were successfully isolated and identified from the rhizosphere soil of sugarcane, and the strain GK-37 had high potassium-releasing ability. Through the optimization of fermentation conditions of GK-37 strain, sucrose was determined as the optimal carbon source. This study is expected to provide a valuable reference for the further development and production of potassium releasing bacteria fertilizer.

Keywords: Agribusiness (search for similar items in EconPapers)
Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:ags:asagre:396302

DOI: 10.22004/ag.econ.396302

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