Early Detection of Mycoplasma Gallisepticum using PCR from Field Samples in Layer Chicken

Mahfuja Khatun, Md. Mostafizer Rahman, Md. Shahidur Rahman Khan, Md. Khaled Hossain and Mir Rawshan Akter

International Journal of Science and Business, 2018, vol. 2, issue 4, 699-706

Abstract: Mycoplasma gallisepticum (MG) is an important avian pathogen which causes significant economic losses within layer poultry industry. To control of this economic losses, early and specific detection is necessary. A total of 48 (tracheal swabs, lungs and air sacs) samples were collected from different infected/suspected flocks causing respiratory distress. DNA was extracted and then, 16sRNA gene was amplified by PCR using species specific primers and identified 6 positive samples (12.5%). MG exhibited from tracheal swab, lung and air sac were 25, 6.25 and 6.25% respectively. Based on result, it is concluded that PCR using tracheal swab can be efficient for rapid screening and early detection of MG in layer chicken.

Keywords: Mycoplasma gallisepticum; Diagnosis; 16S rRNA; PCR; Chicken (search for similar items in EconPapers)
Date: 2018
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