Sample size calculation based on generalized linear models for differential expression analysis in RNA-seq data

Li Chung-I () and Shyr Yu
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Li Chung-I: Department of Statistics, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
Shyr Yu: Center for Quantitative Sciences, Vanderbilt University, 571 Preston Building, Nashville, TN, United States of America

Statistical Applications in Genetics and Molecular Biology, 2016, vol. 15, issue 6, 491-505

Abstract: As RNA-seq rapidly develops and costs continually decrease, the quantity and frequency of samples being sequenced will grow exponentially. With proteomic investigations becoming more multivariate and quantitative, determining a study’s optimal sample size is now a vital step in experimental design. Current methods for calculating a study’s required sample size are mostly based on the hypothesis testing framework, which assumes each gene count can be modeled through Poisson or negative binomial distributions; however, these methods are limited when it comes to accommodating covariates. To address this limitation, we propose an estimating procedure based on the generalized linear model. This easy-to-use method constructs a representative exemplary dataset and estimates the conditional power, all without requiring complicated mathematical approximations or formulas. Even more attractive, the downstream analysis can be performed with current R/Bioconductor packages. To demonstrate the practicability and efficiency of this method, we apply it to three real-world studies, and introduce our on-line calculator developed to determine the optimal sample size for a RNA-seq study.

Keywords: generalized linear models; RNA-seq; sample size calculation (search for similar items in EconPapers)
Date: 2016
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DOI: 10.1515/sagmb-2016-0008

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