Regulation of hydrogen photoproduction in Rhodobacter sphaeroides batch culture by external oxidizers and reducers

Lilit Gabrielyan, Harutyun Sargsyan, Lilit Hakobyan and Armen Trchounian

Applied Energy, 2014, vol. 131, issue C, 20-25

Abstract: Photo-fermentative production of hydrogen (H2) by purple bacterium Rhodobacter sphaeroides MDC6521 from Armenian mineral springs and its regulation by external reducers and oxidizers have been investigated. Reducers such as dl-dithiothreitol (DTT) and dithionite suppress bacterial growth but enhance H2 yield of R. sphaeroides, whereas oxidizer ferricyanide inhibits both processes. The effect of DTT on DCCD-inhibited FoF1-ATPase activity of R. sphaeroides membrane vesicles has been analyzed too. DTT increases the DCCD-inhibited ATPase activity. Thus, more negative values of Eh by addition of DTT might regulate FoF1-ATPase activity. The participation of the ATPase in redox sensing under photo-fermentative H2 production is suggested. This enzyme might be a target, having a significant role in these processes of purple bacteria.

Keywords: Hydrogen photoproduction; Redox potential; Redox reagents; FoF1-ATPase activity; Rhodobacter sphaeroides (search for similar items in EconPapers)
Date: 2014
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Citations: View citations in EconPapers (5)

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DOI: 10.1016/j.apenergy.2014.06.019

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