A thermo- and toxin-tolerant kefir yeast for biorefinery and biofuel production

Jui-Jen Chang, Cheng-Yu Ho, Chi-Tang Mao, Nathan Barham, Yu-Rong Huang, Feng-Ju Ho, Yueh-Chin Wu, Yu-Han Hou, Ming-Che Shih, Wen-Hsiung Li and Chieh-Chen Huang

Applied Energy, 2014, vol. 132, issue C, 465-474

Abstract: Although biorefinery has become a common concept to convert biomass into biofuels and value-added chemicals for better cost-performance, good microbial hosts that can be used to implement the concept are still wanting. In this study, a Kluyveromyces marxianus yeast, named KY3, was isolated from a Taiwanese kefir microbial consortium. We showed that KY3 could grow on a broad spectrum of substrates, including hexose and pentose sugars. It is heat and toxin tolerant, can grow under a wide range of pH values (pH 2.5–9), and shows a high ethanol production rate at elevated temperatures. It also can produce value-added aromatic chemicals, such as 2-phenylethylethanol and 2-phenylethyl acetate, during the fermentative process. A genetic transformation was achieved in KY3 to express a rumen fungal β-glucosidase gene, and the transgenic host (KY3–NpaBGS) could efficiently convert cellobiose to ethanol. Furthermore, it was shown that a novel dual-microbe co-culture system of Bacillus subtilis and KY3–NpaBGS can be employed for bioethanol production from cellulosic material. Thus, KY3 has a high potential to be a good host for biorefinery.

Keywords: Biorefinery; Kefir yeast; Kluyveromyces marxianus; Dual-microbe co-culture; Cellulosic ethanol (search for similar items in EconPapers)
Date: 2014
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0306261914006667
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:eee:appene:v:132:y:2014:i:c:p:465-474

Ordering information: This journal article can be ordered from
http://www.elsevier.com/wps/find/journaldescription.cws_home/405891/bibliographic
http://www.elsevier. ... 405891/bibliographic

DOI: 10.1016/j.apenergy.2014.06.081

Access Statistics for this article

Applied Energy is currently edited by J. Yan

More articles in Applied Energy from Elsevier
Bibliographic data for series maintained by Catherine Liu ().