Equation for isotope accumulation in products and biomass as a way to reveal the pathways in mesophilic methanol methanization by microbial community

V.A. Vavilin

Ecological Modelling, 2010, vol. 221, issue 24, 2881-2886

Abstract: Isotope-labelling of substrate is used to reveal the methabolic pathways of substrate transformation by microbial community. In this paper, in order to describe the batch mesophilic anaerobic methanization of 13C-labelled methanol and microbial ecology analysis (Li et al., 2008), an equation for the isotope accumulation in products and biomass was included into the basic mathematical model based on stoichiometric chemical reactions. The higher was the isotope level in substrate, the larger fraction of 13C accumulated in products and biomass. Acetate, total organic and inorganic carbon (TOC, TIC) concentrations and methane production were used for the model calibration, whereas 13C enrichment of acetate, TIC and biomass were used for model validation. In the model, chemical transformations including methanol and acetate oxidation, homoacetogenesis, hydrogenotrophic and aceticlastic methanogenesis were considered. The rate-limiting reactions were methanol and acetate consumption. According to the model, homoacetogens performing acetate formation and oxidation were competed with hydrogenotrophic methanogens for hydrogen. Biphasic methane production was due to hydrogenotrophic methanogenesis in the first phase and due to acetiticlastic and hydrogenotrophic methanogenesis following acetate oxidation in the second phase.

Keywords: Chemical reactions; 13C; Equation for isotope accumulation; Mesophilic methanol methanization; Microbial community; NanoSIMS (search for similar items in EconPapers)
Date: 2010
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0304380010004606
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:eee:ecomod:v:221:y:2010:i:24:p:2881-2886

DOI: 10.1016/j.ecolmodel.2010.09.004

Access Statistics for this article

Ecological Modelling is currently edited by Brian D. Fath

More articles in Ecological Modelling from Elsevier
Bibliographic data for series maintained by Catherine Liu ().