Concomitant hydrolysis of sucrose by the long half-life time yeast invertase and hydrogen production by the hydrogen over-producing Escherichia coli HD701

Fatthy Mohamed Morsy and Samir Hag Ibrahim

Energy, 2016, vol. 109, issue C, 412-419

Abstract: The hydrogen over-producing Escherichia coli HD701, a hydrogenase up-regulated strain, has the potential for industrial-scale hydrogen production; however, this strain is unable to metabolize sucrose which is a major constituent of many waste organic materials that can be used as feedstock for industrial hydrogen production. Invertase from Sacharomyces cervacea (yeast) was partially purified and characterized where its apparent optimum temperature when using short reaction period (15 min) was 55 °C; however the enzyme couldn't continue active due to its short half-life time at such high temperature. In contrast, a lower optimum temperature (35 °C) was recorded when using long reaction period (5 h) where the enzyme showed long half-life time and stability at such degree of temperature. Consequently, a concomitant hydrolysis of sucrose by yeast invertase and hydrogen production by E. coli at 35 °C was conducted and showed a high potency for industrial application with a hydrogen yield of 0.48 mole hydrogen/mole reducing sugars using batch fermentation at optimum sucrose concentration of 10 g/L. The described approach might be applicable for biotechnologies of other bio-products by E. coli from sucrose as a carbon source.

Keywords: Dark fermentation; Escherichia coli; Hydrogen; Hydrolysis; Sucrose; Yeast invertase (search for similar items in EconPapers)
Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:eee:energy:v:109:y:2016:i:c:p:412-419

DOI: 10.1016/j.energy.2016.05.006

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