Improving xylose utilization and ethanol production from dry dilute acid pretreated corn stover by two-step and fed-batch fermentation

Wen-Chao Li, Xia Li, Jia-Qing Zhu, Lei Qin, Bing-Zhi Li and Ying-Jin Yuan

Energy, 2018, vol. 157, issue C, 877-885

Abstract: It is a great challenge to efficiently utilize xylose in fermentation with high solid loadings for the economically feasible production of lignocellulosic ethanol. However, 20% solid loading of dry dilute acid pretreated corn stover (DDAPCS) decreased 66.3% of the xylose consumption compared to yeast extract peptone dextrose and xylose (YPDX) medium, which was significantly larger than 16% solid loading (9.4%). To overcome those problems, several two-step processes for simultaneous saccharification and co-fermentation (SSCF) were developed by inoculating a water extract of 16% solid loading DDAPCS with microorganisms in the first fermentation step. During the first step, the relatively lower inhibitor concentrations (compared to 20% solid loading), decreased glucose concentration, and the absence of enzymatic hydrolysis residues (EHR) were beneficial for the use of xylose and enabled up to 75.6% of the xylose to be converted to ethanol. With an optimized fed-batch strategy of two-step process, the residual xylose concentration reduced by 55.6% with 20% solid loading, and the maximum ethanol concentration and yield increased by 30% and 16.6%, respectively.

Keywords: Dry dilute acid pretreatment; Xylose utilization; Enzymatic hydrolysis residues; Two-step SSCF; Fed-batch (search for similar items in EconPapers)
Date: 2018
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Citations: View citations in EconPapers (5)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:energy:v:157:y:2018:i:c:p:877-885

DOI: 10.1016/j.energy.2018.06.002

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