Development of control strategies for high cell density cultivations

Reiner Luttmann, Gerhard Bitzer and Jens Hartkopf

Mathematics and Computers in Simulation (MATCOM), 1994, vol. 37, issue 2, 153-164

Abstract: The high cell density cultivation (HCDC) of genetically modified microorganisms, e.g. E. coli, with dry weight larger than 100 g⧸l, requires fed-batch processing with aid of a controlled specific growth rate μ. A general mathematical model of HCDC as well as different methods to control μ is discussed. Furthermore, the implementation of control strategies in an industrial process control system is shortly explained. The test of the control equipment is carried out with by means of on-line simulation methods. The comparison of theoretical investigations with an experimental verification of a high cell density E. coli cultivation demonstrates the the usefulness of our methods.

Keywords: HCDC-high cell density cultivation; Specific growth rate control; pO2-control; General bioreactor process model; On-line simulation, HCDC-control strategies (search for similar items in EconPapers)
Date: 1994
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Persistent link: https://EconPapers.repec.org/RePEc:eee:matcom:v:37:y:1994:i:2:p:153-164

DOI: 10.1016/0378-4754(94)00004-2

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