Electrophoretic separation of proteins via complexation with a polyelectrolyte

E.M. Baskin, B.I. Shklovskii and G.V. Zilberstein

Physica A: Statistical Mechanics and its Applications, 2003, vol. 317, issue 3, 313-320

Abstract: We suggest to augment standard isoelectric focusing for separation of proteins in a gradient of pH by a similar focusing in the presence of a strongly charged polyelectrolyte (PE). Proteins which have the same isoelectric point but different “hidden” charges of both signs in this point make complexes with PE, which focus in different pH. This is a result of charge inversion of such proteins by adsorbed PE molecules, which is sensitive to the hidden charge. Hence, the hidden charge is a new separation parameter.

Keywords: Proteomics; Isoelectric focusing; Charge inversion (search for similar items in EconPapers)
Date: 2003
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Persistent link: https://EconPapers.repec.org/RePEc:eee:phsmap:v:317:y:2003:i:3:p:313-320

DOI: 10.1016/S0378-4371(02)01334-1

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