Detection of ganglioside clustering in DOPC bilayers by 1H-NMR spectroscopy

D’Emiliano, D., C. Casieri, M. Paci and F. De Luca

Physica A: Statistical Mechanics and its Applications, 2007, vol. 374, issue 1, 293-303

Abstract: 1H-NMR spectroscopy has been used to investigate the formation of GM1 clusters in DOPC liposome bilayer. The 1H-DOPC NMR spectra show that the presence of GM1 modifies some resonance lines. These modifications are imputable to the interaction of DOPC with GM1. Above a certain GM1 concentration the trend of such distortions suggests the formation of GM1 aggregates. This work provides a new and simple NMR proof of the GM1 clustering in DOPC liposomes and therefore on model cell membranes; it allows to estimate the mean size of GM1 aggregates and the GM1 concentration at which clustering starts. In addition, the behaviour of the 1H-NMR line-width shows that the presence of GM1 modifies the bilayer arrangement making it more mobile, especially at GM1-clustering concentration.

Keywords: Lipid rafts; 1H-NMR spectroscopy; Chemical shielding (search for similar items in EconPapers)
Date: 2007
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Persistent link: https://EconPapers.repec.org/RePEc:eee:phsmap:v:374:y:2007:i:1:p:293-303

DOI: 10.1016/j.physa.2006.07.021

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