Enhancement of acetone-butanol-ethanol fermentation from eucalyptus hydrolysate with optimized nutrient supplementation through statistical experimental designs

Jin Zheng, Yukihiro Tashiro, Tao Zhao, Qunhui Wang, Kenji Sakai and Kenji Sonomoto

Renewable Energy, 2017, vol. 113, issue C, 580-586

Abstract: Eucalyptus has been previously suggested as a potential substrate for acetone-butanol-ethanol (ABE) production without nutrient supplementation; however, incomplete sugar consumption has prevented improvement of ABE production. Cellulase loading with 35 FPU g−1 was first optimized in terms of high hydrolysis efficiency (95%). However, only 0.43 g L−1 ABE production and 3.44% glucose consumption rate were achieved. To improve ABE production from eucalyptus hydrolysate, supplementation of 6 nutrients in common tryptone-yeast extract (TY) medium were investigated by statistical approaches. Three nutrients including yeast extract, tryptone, and FeSO4·7H2O were screened as significant nutrients for ABE production. Subsequently, use of a modified TY medium (MTY medium: yeast extract 3.04 g L−1, tryptone 7.64 g L−1, FeSO4·7H2O 15.3 mg L−1), which was subsequently predicted by Plackett-Burman and Box-Behnken designs to stimulate ABE production, resulted in ca. 40-fold increase in ABE concentration (16.9 g L−1) and a glucose consumption rate of 100%. We first examined previously uninvestigated nutrition combinations using Plackett-Burman and Box-Behnken designs for high ABE production from eucalyptus hydrolysate. This study shows that statistical method would be a powerful tool for the optimization and enhancement of ABE production from eucalyptus hydrolysate.

Keywords: Acetone-butanol-ethanol fermentation; Cellulase loading; Significant nutrient; Statistical experimental design (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:113:y:2017:i:c:p:580-586

DOI: 10.1016/j.renene.2017.05.097

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