A mild extraction and separation procedure of polysaccharide, lipid, chlorophyll and protein from Chlorella spp

Wenhuan Zhao, Meirong Duan, Xu Zhang and Tianwei Tan

Renewable Energy, 2018, vol. 118, issue C, 701-708

Abstract: This study developed a way to separate lipid, polysaccharide, protein and chlorophyll from wet Chlorella spp. (85% moisture content, wt) by using three-phase partitioning (TPP). The optimum conditions for the key steps were researched and defined as (I) cell disruption at the following conditions: biomass concentration 20 g/L, bath temperature 90 °C, duration 2.5 h, enzyme concentration 12 g/L, enzyme hydrolysis time 4 h; and (II) TPP at 40% (NH4)2SO4 (wt, %), sample solution/t-butanol 1: 2 (V/V), room temperature. The extraction yield of lipid and the poysaccharide preservation rate by the optimized procedure were 80.24%, 90.63%, respectively. The protein removal rate was 78.87%. The results provided a promising towards the industrial separation of lipid, polysaccaride, protein and chlorophyll.

Keywords: Chlorella spp.; Polysaccharides; Lipid; Protein; Chlorophyll (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:118:y:2018:i:c:p:701-708

DOI: 10.1016/j.renene.2017.11.046

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