Evaluation of transcription profile of acetyl-CoA carboxylase (ACCase) and acyl-ACP synthetase (AAS) to reveal their roles in induced lipid accumulation of Synechococcus sp. HS01

Sima Modiri, Hossein Shahbani Zahiri, Hojatollah Vali and Kambiz Akbari Noghabi

Renewable Energy, 2018, vol. 129, issue PA, 347-356

Abstract: It was earlier shown that the mixotrophic cultivation of Synechococcus sp. HS01 in ostrich oil-containing BG11 medium leads to an up to 2.82- fold increase in lipid productivity compared with autotrophic condition. A follow-up investigation was carried out to divulge whether conditions that would increase the lipid content in Synechococcus sp. HS01, bringing about constantly more pool towards the main route for fatty acids synthesis. To this end, the expression patterns of two genes including acc as the main enzyme involved in fatty acid synthesis pathway, converting Acetyl-CoA to Malonyl-CoA and aas gene which convert free fatty acids to active Acyl carrier protein (ACP) (through esterification) were carefully analyzed under different culture conditions.

Keywords: Synechococcus sp. HS01; Ostrich oil; Lipid content; Mixotrophic cultivation; Transcription analysis; De novo fatty-acid synthesis (search for similar items in EconPapers)
Date: 2018
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Citations: View citations in EconPapers (1)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:129:y:2018:i:pa:p:347-356

DOI: 10.1016/j.renene.2018.06.002

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