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An experimental and modeling approach for ethanol production by Kluyveromyces marxianus in stirred tank bioreactor using vacuum extraction as a strategy to overcome product inhibition

Bruna Tavares, Maria das Graças de Almeida Felipe, Júlio César dos Santos, Félix Monteiro Pereira, Simone Damasceno Gomes and Luciane Sene

Renewable Energy, 2019, vol. 131, issue C, 261-267

Abstract: Vacuum ethanol removal effect on the fermentative performance of Kluyveromyces marxianus was assessed. Yeast was cultured in bench bioreactor at initial glucose concentration of 90 g L−1 in two sequential batches of 36 h, with a vacuum ethanol removal step and glucose feed before the second batch. Ethanol concentration after the first batch was 34.13 g L−1 (YP/S 0.38 g g−1; QP 0.94 g L−1.h−1), and 40.90 g L−1 (YP/S 0.18 g g−1; QP 0.43 g L−1.h−1) after the second batch. Ethanol production in a control assay without vacuum extraction only happened in the first batch (36.37 g L−1; YP/S 0.4 g g−1; QP 1.01 g.L−1h−1). The phenomenological modeling showed product inhibition and the importance of product removal to process improvement, although the yeast had needed an adaptation period to recover its fermentative metabolism after the stress caused by vacuum.

Keywords: Ethanol; Product inhibition; Product removal; Vacuum; Phenomenological modeling (search for similar items in EconPapers)
Date: 2019
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:131:y:2019:i:c:p:261-267

DOI: 10.1016/j.renene.2018.07.030

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