The optimized co-cultivation system of Penicillium oxalicum 16 and Trichoderma reesei RUT-C30 achieved a high yield of hydrolase applied in second-generation bioethanol production

Xihua Zhao, Shi Yi and Hanxin Li

Renewable Energy, 2019, vol. 136, issue C, 1028-1035

Abstract: A low-level secretion of hydrolase and low conversion efficiency represent two major challenges in production of second-generation bioethanol. In the study, the co-cultivation system of Penicillium oxalicum 16 and Trichoderma reesei RUT-C30 optimized by response surface methodology under solid state fermentation produced 38.0 IU/gds, 352.9 IU/gds, 713.2 IU/gds, 15.7 IU/gds and 188.6 IU/gds for FPase, xylanase, amylase, cellobiohydrolase and β-1, 4-glucosidase, respectively, which was corresponding to 4.2 fold, 2.9 fold, 2.03 fold, 1.08 fold and 1.96 fold higher than that without optimization. Moreover, unpretreated wheat bran which was hydrolyzed by the optimized co-cultivation system and fermented by Saccharomyces cerevisiae UV-20 was converted into 26.8 g/L ethanol corresponding to 98.41% of the conversion rate, and produced much more ethanol than milled rice straw. The study provided a feasible method which can enhance hydrolase yield and very efficiently produce second-generation bioethanol.

Keywords: Hydrolase; Second-generation bioethanol; Co-cultivation; Solid state fermentation; Penicillium oxalicum 16; Trichoderma reesei RUT-C30 (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:136:y:2019:i:c:p:1028-1035

DOI: 10.1016/j.renene.2019.01.066

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