Improving co-fermentation of glucose and xylose by adaptive evolution of engineering xylose-fermenting Saccharomyces cerevisiae and different fermentation strategies

Wen-Chao Li, Jia-Qing Zhu, Xiong Zhao, Lei Qin, Tao Xu, Xiao Zhou, Xia Li, Bing-Zhi Li and Ying-Jin Yuan

Renewable Energy, 2019, vol. 139, issue C, 1176-1183

Abstract: Xylose utilization of engineered yeast is vulnerable to inhibitors generated during pretreatment of lignocellulose. In this study, adaptive evolution was applied to enhance the tolerance of xylose-fermenting strain. Compared to the parental strain, the ethanol yield was increased by 60% and 80% for the adapted strain (E7-403) when xylose was used as the sole carbon resource with 20% and 50% inhibitor cocktails, respectively. E7-403 removed furfural more effectively than parental strain (E7) in the fermentation with 100% inhibitor cocktails. In the fermentation with mixed sugar and high inhibitor concentration, glucose was depleted within 36 h for E7-403 while 6.1 g/L glucose was still left after 120 h for E7. Consequently, ethanol yield of E7-403 was 22.9% higher than that of E7. It was demonstrated that E7-403 strain exhibited an enhanced ability for regulating cellular reactive oxygen species, which alleviated the harmful effects of inhibitors. Meanwhile, E7-403 strain was further applied in co-culture and pre-fermentation process to improve xylose utilization.

Keywords: Tolerance to multiple inhibitors; Adaptive evolution; Reactive oxygen species; Co-culture fermentation; Simultaneous saccharification and co-fermentation (search for similar items in EconPapers)
Date: 2019
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Citations: View citations in EconPapers (9)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:139:y:2019:i:c:p:1176-1183

DOI: 10.1016/j.renene.2019.03.028

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