Enhanced photo-fermentative hydrogen production by synergistic effects of formed biofilm and added L-cysteine

Han-Quan Wen, De-Feng Xing, Guo-Jun Xie, Tian-Ming Yin, Nan-Qi Ren and Bing-Feng Liu

Renewable Energy, 2019, vol. 139, issue C, 643-650

Abstract: In this work, the synergistic effects of L-cysteine and biofilm on the performance of reactor were investigated in the photo-fermentative biofilm reactor. Both L-cysteine and biofilm could enhance the performance of reactor for producing hydrogen. The optimal L-cysteine concentrations in the biofilm reactor (BR) and control reactor (CR, reactor with no biofilm) were 0.5 g/L and 0.75 g/L, respectively. In addition, the maximum cumulative hydrogen volume (3500 ± 170 mL/L), substrate conversion efficiency (79.4 ± 4.0%) and hydrogen yield 867 ± 43 mL/g (acetate) were obtained in the BR at L-cysteine concentration of 0.5 g/L. It is intriguing that extracellular DNA (eDNA) occupied 50% of EPS, which could play a key role in hydrogen production. Biofilm could decrease EPS concentration of cells in the BR at each L-cysteine concentration to divert more energy into hydrogen. The moderate L-cysteine concentration could improve bacterial growth and regulate the EPS to promote the biofilm formation and enhance hydrogen production. Thus, the regulation of L-cysteine concentration will be a practical method to enhance hydrogen production by the biofilm reactor.

Keywords: Photo-fermentative hydrogen production; L-cysteine; Biofilm; EPS; eDNA (search for similar items in EconPapers)
Date: 2019
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Citations: View citations in EconPapers (1)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:139:y:2019:i:c:p:643-650

DOI: 10.1016/j.renene.2019.02.127

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