Sugarcane bagasse saccharification using Aspergillus tubingensis enzymatic cocktail for 2G bio-ethanol production

Bhanu Pratap Prajapati, Uttam Kumar Jana, Rahul Kumar Suryawanshi and Naveen Kango

Renewable Energy, 2020, vol. 152, issue C, 653-663

Abstract: In order to meet the increasing demand of 2G ethanol, sugarcane bagasse (SCB), a low cost by-product of sugarcane industry, can be utilized. However, cost-effective degradation of SCB into constituent fermentable sugars and subsequent ethanolic fermentation remains to be a far-fetched goal. In the present study, cellulase and hemicellulase cocktail produced by Aspergillus tubingensis NKBP-55 was applied for SCB hydrolysis under parametrically optimized conditions. Enzyme treatment (SCB: 7%, enzyme load: 1U = 1 mg) resulted in the liberation of fermentable mono-sugars 20 mg/mL (glucose, xylose and arabinose). Powder X-ray diffraction (PXRD) analysis revealed increase in the crystallinity index from 58.4 to 63.9% as a result of degradation of hemicellulose and amorphous cellulose. SCB hydrolysate was fermented to ethanol (0.415 g/g) by glucose and xylose-fermenting yeast, Candida shehatae NCIM 3501. Maximum ethanol concentration (15.54 ± 0.3 g/L) with 77.9% fermentation efficiency and 0.161 g/L/h productivity was achieved. The present study revealed that A. tubingensis enzyme cocktail can be used for efficient SCB hydrolysis and conversion of resulting hydrolysate into bioethanol using C. shehatae.

Keywords: Aspergillus tubingensis NKBP-55; Bioethanol; Candida shehatae; Cellulase; Ethanolic co-fermentation; Sugarcane bagasse (search for similar items in EconPapers)
Date: 2020
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Citations: View citations in EconPapers (4)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:152:y:2020:i:c:p:653-663

DOI: 10.1016/j.renene.2020.01.063

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