Construction of multi-enzyme cascade biomimetic carbon sequestration system based on photocatalytic coenzyme NADH regeneration

Junhui Zhou, Senshen Yu, Helong Kang, Rui He, Yuxin Ning, Yingyue Yu, Meng Wang and Biqiang Chen

Renewable Energy, 2020, vol. 156, issue C, 107-116

Abstract: CO2 is one of the main gases leading to the greenhouse effect. The use of a multi-enzyme biological carbon capture and utilization method can reduce the concentration of CO2. However, most oxidoreductases require NADH as a coenzyme, which is too expensive to afford for most situation. In this research, NADH was regenerated using photocatalysis and CO2 was converted to formaldehyde by FateDH and FaldDH gradually. TCPP and ZIF-8 were successfully combined to construct a photocatalytic multi-enzyme cascade biomimetic carbon sequestration system for the first time. TCPP was used as the photocatalyst and ZIF-8 was used as the multi-enzyme immobilized carrier for FateDH and FaldDH. The TCPP content, material dosage, pH of reaction solution and the ratio of the two enzymes were optimized in this study. The results indicated that when reacting under visible light for 3 h, using 1 mg/mL 3% TCPP @ZIF-8 at pH 8.0 PBS condition, it has the highest NADH reduction rate, reaching 75.04%. It was also found that when FateDH: FaldDH = 2:1, the formaldehyde yield can reach up to 7.74 μM. And the formaldehyde conversion rate reached 77.37%. In addition, this composite system retained 52.93% of residual activity after 10 batches of re-use.

Keywords: Biological carbon fixation; Photocatalysis; NADH regeneration; Multiple enzyme fixation; Multiple enzyme cascade (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0960148120305504
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:156:y:2020:i:c:p:107-116

DOI: 10.1016/j.renene.2020.04.022

Access Statistics for this article

Renewable Energy is currently edited by Soteris A. Kalogirou and Paul Christodoulides

More articles in Renewable Energy from Elsevier
Bibliographic data for series maintained by Catherine Liu ().