Isobutanol production from empty fruit bunches

Jungwoo Yang, Jae Kyun Kim, Jung-Oh Ahn, Young-Ha Song, Chul-Soo Shin, Yong-Cheol Park and Kyoung Heon Kim

Renewable Energy, 2020, vol. 157, issue C, 1124-1130

Abstract: Although isobutanol has been successfully produced from glucose-based media by various microbes, only a few studies have reported isobutanol production from lignocellulosic biomass. In this study, the feasibility of producing isobutanol from hydrothermally-pretreated empty fruit bunches (EFBs) was analyzed. Firstly, the Escherichia coli JK209 strain was constructed; subsequently, optimal fermentation conditions for isobutanol production using this strain were determined. Next, the raw EFBs were hydrothermally pretreated at 190 °C for 15 min, and the pretreated EFBs were hydrolyzed using 10 FPU of cellulase/g glucan. Finally, the resulting hydrolysate was fermented at 25 °C for 84 h, and isobutanol give rise to the yield of 0.26 g isobutanol/g glucose. These results imply that 80.1 g of isobutanol could be produced from 1 kg dry weight of EFBs. To our knowledge, this is the first feasibility study of isobutanol production from pretreated EFBs.

Keywords: Isobutanol; Empty fruit bunches; Lignocellulose; Pretreatment; Separate hydrolysis and fermentation (search for similar items in EconPapers)
Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:157:y:2020:i:c:p:1124-1130

DOI: 10.1016/j.renene.2020.05.067

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