Metabolomics reveals a lipid accumulation mechanism involving carbon allocation in Scenedesmus obliquus under norfloxacin stress

Huaqiang Chu, Li Ren, Libin Yang, Jiabin Chen, Xuefei Zhou and Yalei Zhang

Renewable Energy, 2020, vol. 157, issue C, 585-592

Abstract: The transformation of organic components and the mechanism of lipid accumulation under stress in an algae–antibiotic system were studied. It was found that the antibiotic norfloxacin (NOR; 5–80 mg/L) had a pronounced effect on the lipid accumulation of the cultured Scenedesmus obliquus. Upon exposure to 80 mg/L NOR, the lipid content of the algae cells increased to 30.8%, which was approximately 2-fold higher than that of the blank control culture. Metabolomics was used to identify the biochemical changes induced by NOR in S. obliquus. The tricarboxylic acid cycle and the metabolic pathways of fatty acids, starch, and amino acids significantly changed under NOR stress. This stress triggered the production of reactive oxygen species, which contributed to intracellular signaling. This led to the shifting of additional common precursors (such as pyruvate and glucose) from the starch pathway to the fatty acid synthesis pathway and resulted in increased lipid accumulation and carbohydrate downregulation. Hence, carbon allocation shifted from carbohydrates to lipids as a storage reserve.

Keywords: Wastewater; Algae cultivation; Antibiotic; Lipid accumulation; Carbon allocation (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0960148120307497
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:157:y:2020:i:c:p:585-592

DOI: 10.1016/j.renene.2020.05.051

Access Statistics for this article

Renewable Energy is currently edited by Soteris A. Kalogirou and Paul Christodoulides

More articles in Renewable Energy from Elsevier
Bibliographic data for series maintained by Catherine Liu ().