Enhanced microalgae biomass and lipid output for increased biodiesel productivity

Keli C.C. Morais, Daniele Conceição, José V.C. Vargas, David A. Mitchell, André B. Mariano, Juan C. Ordonez, Lygia Vitoria Galli-Terasawa and Vanessa M. Kava

Renewable Energy, 2021, vol. 163, issue C, 138-145

Abstract: This paper investigated the cumulative impact of salinity, carbon source (glycerol and glucose) and photoperiod on the cultivation of the microalga Phaeodactylum tricornutum in mixotrophic growth in pure air supplied photobioreactors aiming at biomass output and lipid content enhancement for increased biodiesel productivity. For that, Phaeodactylum tricornutum was grown for 18 and 20 days in modified F2 medium which was supplied in the beginning of the culture, and with daily addition of glycerol (0.1 M) or glucose (0.05 M); salinity at 15 and 30‰, with the luminosity of 165 μmols photons m−2s−2 for 24 h day−1 or partial 12 h day−1. Biomass production was registered, total lipids quantified with the Bligh and Dyer methodology, and the lipids’ drops observed with Nile Red staining. Regarding salinity, a value of 15‰ led to the highest microalgae growth. Glycerol 0.1 M was the carbon source which provided the best assimilation by the microalgae, reaching up to 1.3 g L−1 of biomass. The 24 h-illumination photoperiod with glycerol in mixotrophic cultivation led to 338.97 mg L−1 of lipids, which was roughly 80% higher than the lipid content obtained with autotrophic growth. In conclusion, the most effective conditions were glycerol 0.1 M (carbon source), 15‰ salinity, and 24 h-illumination.

Keywords: Microalgae; Glycerol; Lipids; TAG; Mixotrophic; Autotrophic (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0960148120313276
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:163:y:2021:i:c:p:138-145

DOI: 10.1016/j.renene.2020.08.082

Access Statistics for this article

Renewable Energy is currently edited by Soteris A. Kalogirou and Paul Christodoulides

More articles in Renewable Energy from Elsevier
Bibliographic data for series maintained by Catherine Liu ().