One-step direct transesterification of wet yeast for biodiesel production catalyzed by magnetic nanoparticle-immobilized lipase

Xiyue Cao, Hui Xu, Fosheng Li, Yijun Zou, Yulu Ran, Xiaorui Ma, Yu Cao, Qingrui Xu, Dairong Qiao and Yi Cao

Renewable Energy, 2021, vol. 171, issue C, 11-21

Abstract: To develop a method for direct transesterification of wet yeast using immobilized lipase, the oleaginous yeast Saitozyma podzolica Zwy-2-3 and the lipase producing Burkholderia pyrrolica WZ10-3 were used as materials for production of biodiesel. Fe3O4@SiO2-CHO prepared by modifying Fe3O4 with TEOS, APTES and glutaraldehyde. The biocatalysts covalently cross-linked with WZ10-3 lipase by Fe3O4@SiO2-CHO were characterized by FTIR, XRD and TEM. When the enzyme dosage, glutaraldehyde concentration, temperature and time were 30.22 mL, 2.0%, 40 °C and 4 h, the immobilized lipase activity and immobilization rate reached 10038.0 U/g and 96.9%, respectively. The optimum temperatures for immobilized and free lipase were 60 °C and 40 °C. The immobilized enzyme still had 80% enzymatic activity after 48 d storage at 4 °C. The optimized conditions for the direct conversion of immobilized lipase to esterified wet yeast (one-step) were: enzyme dosage 2.5 g, reaction temperature 35 °C; water content 15%; and molar ratio of n-hexane to methanol 3: 1. The transesterification rates of one-step method for oil and biomass were 98.12% and 56.11%, respectively. In contrast, the two-step method was only 88.75% and 51.21%. The immobilized enzyme had 90% enzyme activity after 10 times of reuse.

Keywords: Direct transesterification; Biodiesel; Immobilized lipase; Magnetic nanomaterials; Saitozyma podzolica; Burkholderia pyrrolica (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (4)

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0960148121002342
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:171:y:2021:i:c:p:11-21

DOI: 10.1016/j.renene.2021.02.065

Access Statistics for this article

Renewable Energy is currently edited by Soteris A. Kalogirou and Paul Christodoulides

More articles in Renewable Energy from Elsevier
Bibliographic data for series maintained by Catherine Liu ().