Flow cytometry assay to evaluate lipid production by the marine microalga Tetraselmis sp. using a two stage process

Mouna Dammak, Hajer Ben Hlima, Fatma Elleuch, Chantal Pichon, Philippe Michaud, Imen Fendri and Slim Abdelkafi

Renewable Energy, 2021, vol. 177, issue C, 280-289

Abstract: Tetraselmis sp. strain MD-01, a microalga isolated from the Tunisian marine coast, has been studied for autotrophic lipids production with two stage process. This strategy was conducted for maximum cells production under moderated conditions (2.12 g L−1), followed by cultivation under stressed conditions to induce lipid synthesis. High irradiance (300 μmol m−2 s−1) and nitrogen depletion (S mode) showed better carotenoids accumulation and lower chlorophylls content in microalgae compared to those obtained in biomass cultivated under high irradiance (Sg mode). This two-stage strategy allowed to increase of lipid content in cells cultivated in Sg and S modes from 18% DW (stage I) to 50 and 61.5% DW (stage II), respectively, with an increase of saturated and long chain fatty acids levels. Based on fluorometry analyses, an increase of lipids in Nile-Red stained cells and a decrease of chlorophylls auto-fluorescence under stressed conditions were shown. From spectral data, the lipids: amide I ratio obtained from S mode was 2.17 and 4.45 times higher than that from Sg mode and control conditions, respectively. These results revealed the potential of Tetraselmis strain MD-01 for biodiesel production.

Keywords: Tetraselmis; Two-stage process; Lipids; High irradiance; Nitrogen depletion (search for similar items in EconPapers)
Date: 2021
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Citations: View citations in EconPapers (2)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:177:y:2021:i:c:p:280-289

DOI: 10.1016/j.renene.2021.05.126

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