Biological production and recovery of 2,3-butanediol using arabinose from sugar beet pulp by Enterobacter ludwigii

Vivek Narisetty, Sudheera Narisetty, Samuel Jacob, Deepak Kumar, Gary A. Leeke, Anuj Kumar Chandel, Vijai Singh, Vimal Chandra Srivastava and Vinod Kumar

Renewable Energy, 2022, vol. 191, issue C, 394-404

Abstract: Sugar beet pulp (SBP) is a major byproduct from the sugar industries and consists of >20% w/w arabinose. The current work evaluated the potential of Enterobacter ludwigii assimilating pure arabinose and arabinose rich hydrolysate from SBP pellets for 2,3-butanediol (BDO) production. The hydrolysate was obtained through dilute acid pretreatment (DAP) with sulphuric acid. The process was optimized for acid and solid loading to obtain a hydrolysate free from furan derivatives. The effect of different levels of substrate (10–60 g/L) using pure arabinose was conducted in shake flask experiments, followed by co-fermentation with small amounts of glucose and SBP hydrolysate. After flask cultivations, BDO fermentations were carried-out in a bench-top bioreactor in batch and fed-batch modes using pure arabinose as well as SBP hydrolysate. The fed-batch culture led to BDO production of 42.9 and 35.5 g/L from pure arabinose and SBP hydrolysate with conversion yields of 0.31 and 0.29 g/g, respectively. Finally, BDO accumulated on pure arabinose and SBP hydrolysate were recovered using an aqueous two-phase extraction system. The recovery yield of BDO accumulated on arabinose and hydrolysate was ∼97%. The work demonstrated the feasibility of using SBP as a suitable feedstock for manufacturing BDO.

Keywords: 2,3-Butanediol; Arabinose; Sugar beet pulp; Pretreatment; Aqueous two-phase extraction system (search for similar items in EconPapers)
Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:191:y:2022:i:c:p:394-404

DOI: 10.1016/j.renene.2022.04.024

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