Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production

Xuemin Wang, Yanan Wang, Qiaoning He, Yantao Liu, Man Zhao, Yi Liu, Wenting Zhou and Zhiwei Gong

Renewable Energy, 2022, vol. 197, issue C, 1133-1143

Abstract: High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes.

Keywords: Cutaneotrichosporon oleaginosum; Corn stover; Fed-batch saccharification; Fed-batch fermentation; Microbial lipid; Biodiesel (search for similar items in EconPapers)
Date: 2022
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Citations: View citations in EconPapers (1)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:197:y:2022:i:c:p:1133-1143

DOI: 10.1016/j.renene.2022.08.002

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