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Biological conversion of methane to methanol at high H2S concentrations with an H2S-tolerant methanotrophic consortium

Danping Jiang, Xumeng Ge, Long Lin, Zhou Chen, Quanguo Zhang and Yebo Li

Renewable Energy, 2023, vol. 204, issue C, 475-484

Abstract: To develop biological biogas to methanol conversion technology without costly hydrogen sulfide (H2S) removal, a high H2S tolerant methanotrophic consortium (HTMC), was enriched from an anaerobic digester effluent in presence of 5.64 g/m3 H2S in the gas phase. The HTMC can grow stably and produce methanol under conditions with CH4/air mixtures containing 5.64 g/m3 of H2S. There is no significant (p > 0.05) difference in cell yield or CH4 to methanol conversion efficiency between trials with different H2S concentrations from 0 g/m3 to 5.64 g/m3. Under optimal conditions, a cell yield of 0.333 g cells/g CH4, a methanol concentration of 0.28 mg/mL, and a CH4 to methanol conversion efficiency of 0.22 mol/mol were obtained, respectively. Besides methanotrophs (14.85%) and other bacteria, Cyanobacteria were also identified in the HTMC with a high abundancy (32.16%), which could broaden the application of HTMC for simultaneous utilization of CH4 and CO2 from raw biogas.

Keywords: Methanotrophic consortium; Hydrogen sulfide; Biological conversion; Methanol production (search for similar items in EconPapers)
Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:204:y:2023:i:c:p:475-484

DOI: 10.1016/j.renene.2022.12.106

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