Insights of Pichia kudriavzevii SVMS2019 for cellulase production and fermentation into ethanol

Merlin Sobia Poomani, Iyyadurai Mariappan, Krishnaveni Muthan and Venkatesh Subramanian

Renewable Energy, 2024, vol. 225, issue C

Abstract: The use of lignocellulosic biomass to produce value-added products, such as fuel ethanol, is a current priority for global biobased economy. The quest to explore novel microbes, such as yeasts, to attain sustainable products for an ever-growing population has become highly in demand. This study might be the first to explore the rumen yeast Pichia kudriavzevii SVMS2019 for cellulase production to efficiently utilize wheat straw as biomass, which can further be converted to bioethanol. The optimization process was carried out on pre-treated wheat straw by employing one factor at a time (OFAT) and response surface methodology (RSM). Wheat straw pre-treated using NaOH alkali pretreatment was characterized with FTIR, XRD, and SEM studies. The findings revealed that the P. kudriavzevii SVMS2019 obtained maximum cellulase activity of 44.064 IU/ml at 37.5 °C, pH 3.5, 120 h, and 1.5% of NaOH pre-treated wheat straw (substrate concentration) through RSM. It exhibited 1.25-fold increased production compared to OFAT. The stability of crude cellulase was observed at 45 °C and pH 6. Further, the hydrolyzed reducing sugars produced under optimal conditions opted for fermentation, with a maximum bioethanol content of 12.13 g/l obtained at 37.5 °C in a 24 h interval.

Keywords: Cellulose; Production optimization; NaOH pretreatment; Rumen yeast; Wheat straw (search for similar items in EconPapers)
Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:225:y:2024:i:c:s0960148124003616

DOI: 10.1016/j.renene.2024.120296

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