A combined immobilization system for high-solids cellulosic ethanol production by simultaneous saccharification and fermentation

Dongdong Kong, Zhiling Chen, Han Liu, Xiaoting Qi, Yanping Liu, Shi Xu, Yutong Ye, Juanjuan Li, Peiqi Liang, Shan Wang, Wenhao Hu, Xinyu Jin, Xinchun Liu, Cheng Zhang and Shen Tian

Renewable Energy, 2025, vol. 241, issue C

Abstract: A novel combined immobilization technology was introduced in this work to improve the efficiency and stability of a high-solids biocatalysis system. First, a nano biocatalyst based on commercial cellulase immobilized onto the Fe3O4@SiO2-APTES nanoparticles was synthesized utilizing glutaraldehyde as a chemical crosslinking agent with a final immobilization efficiency of 93.0 %. A decrease in the Vmax and Km values indicates that the nanoparticle-immobilized cellulase enzyme has an increased binding affinity for the cellulose substrate. Next, a recombinant yeast strain was constructed via yeast cell-surface immobilization with laccase and versatile peroxidase for synergistic lignin-degradation. Functional accessibility was detected by using flow cytometry and immunofluorescence microscopy. Finally, the cooperative biocatalysis of nanoparticle-immobilized cellulase and cell-surface immobilized S. cerevisiae strain for SSF were investigated. Fed-batch operation of the solids at a 30.0 % (DW, w/w) final substrate loading was implemented to manage the instantaneous concentration level of inhibitors and enhance the saccharification of undetoxified biomass. The combined immobilization produced a maximum ethanol titer of 79.5 ± 4.3 g/L and a theoretical ethanol yield of 88.2 % with a low cellulase loading of 10 FPU/g cellulose. This work presented a promising green pathway for achieving efficient cellulosic ethanol production, highlighting potential industrial applications and contributing to manufacturing optimization.

Keywords: Immobilization; Steam-exploded corn stover; Lignin degradation; Cell surface display; Simultaneous saccharification and fermentation (search for similar items in EconPapers)
Date: 2025
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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:241:y:2025:i:c:s0960148124023723

DOI: 10.1016/j.renene.2024.122304

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