Production of ethanol from cassava pulp via fermentation with a surface-engineered yeast strain displaying glucoamylase

Akihiko Kosugi, Akihiko Kondo, Mitsuyoshi Ueda, Yoshinori Murata, Pilanee Vaithanomsat, Warunee Thanapase, Takamitsu Arai and Yutaka Mori

Renewable Energy, 2009, vol. 34, issue 5, 1354-1358

Abstract: Cassava (Manihot esculenta Crantz) pulp, produced in large amounts as a by-product of starch manufacturing, is a major biomass resource in Southeast Asian countries. It contains abundant starch (approximately 60%) and cellulose fiber (approximately 20%). To effectively utilize the cassava pulp, an attempt was made to convert its components to ethanol using a sake-brewing yeast displaying glucoamylase on the cell surface. Saccharomyces cerevisiae Kyokai no. 7 (strain K7) displaying Rhizopus oryzae glucoamylase, designated strain K7G, was constructed using the C-terminal-half region of α-agglutinin. A sample of cassava pulp was pretreated with a hydrothermal reaction (140°C for 1h), followed by treatment with a Trichoderma reesei cellulase to hydrolyze the cellulose in the sample. The K7G strain fermented starch and glucose in pretreated samples without addition of amylolytic enzymes, and produced ethanol in 91% and 80% of theoretical yield from 5% and 10% cassava pulp, respectively.

Keywords: Cassava pulp; Glucoamylase; Saccharomycescerevisiae; Arming yeasts; Ethanol; Surface-engineering (search for similar items in EconPapers)
Date: 2009
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Citations: View citations in EconPapers (9)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:34:y:2009:i:5:p:1354-1358

DOI: 10.1016/j.renene.2008.09.002

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