 Effect of heat pre-treatment temperature on isolation of hydrogen producing functional consortium from soilAnita Ravindran, Sunil Adav and Shang-Shyng Yang Renewable Energy, 2010, vol. 35, issue 12, 2649-2655 Abstract: A functional hydrogen producing consortium was isolated from soil by heat pre-treatment technique and hydrogen production at different substrate concentration was evaluated. The forest soil was heat pre-treated at 65, 80, 95, 105 and 120 °C temperature for 1 h. As revealed by PCR-DGGE analysis and hydrogen yield, the hydrogen producing microbial community changed with increase in heat pre-treatment temperatures giving potential hydrogen producing consortium at 95–105 °C soil pre-treatment. The maximum hydrogen production rate, hydrogen yield and cumulative hydrogen with 15–20 g glucose were 1390–1576 mL/L/day, 1.83–1.93 mol H2/mol glucose, and 2966–3146 mL H2/L, respectively. The metabolic pathways shifted from ethanol-type to acetate–formate type as soil pre-treatment temperature increased from 65 to 120 °C. The soil heat pre-treatment approach is effective for isolating hydrogen producing natural Clostridium consortium from the soil as enumerations of the functional strains need specific temperature range to florish. Keywords: Hydrogen production; Functional consortium; Heat pre-treatment; Forest soil (search for similar items in EconPapers) Downloads: (external link) Related works: Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:35:y:2010:i:12:p:2649-2655 DOI: 10.1016/j.renene.2010.04.010 Access Statistics for this article Renewable Energy is currently edited by Soteris A. Kalogirou and Paul Christodoulides More articles in Renewable Energy from Elsevier |
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