Feasibility of biohydrogen production from tofu wastewater with glutamine auxotrophic mutant of Rhodobacter sphaeroides

G.H. Zheng, L. Wang and Z.H. Kang

Renewable Energy, 2010, vol. 35, issue 12, 2910-2913

Abstract: NH4+, which is normally the integrant in organic wastewater, such as Tofu wastewater, is an inhibitor to hydrogen production by anoxygenic phototrophic bacterium. In order to release inhibition of NH4+ to biohydrogen generation by Rhodobacter sphaeroides, a glutamine auxotrophic mutant R. sphaeroides TJ-0803 was obtained by mutagenizing with ethyl methane sulfonate. The mutant could generate biohydrogen efficiently in the medium with high NH4+ concentration, because the inhibition of NH4+ to nitrogenase was released. Under suitable conditions, TJ-0803 could effectively produce biohydrogen from tofu wastewater, which commonly containing 50–60 mg L−1 NH4+, and the generation rate was increased by more than 100% compared with that from wild-type R. sphaeroides.

Keywords: Ammonium; Anoxygenic phototrophic bacterium; Biohydrogen; Glutamine auxotrophic; Tofu wastewater (search for similar items in EconPapers)
Date: 2010
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Citations: View citations in EconPapers (7)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:35:y:2010:i:12:p:2910-2913

DOI: 10.1016/j.renene.2010.04.030

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