Recombinant multi-functional cellulase activity in submerged fermentation of lignocellulosic wastes

Peizhou Yang, Liqiong Guo, Shujie Cheng, Nannan Lou and Junfang Lin

Renewable Energy, 2011, vol. 36, issue 12, 3268-3272

Abstract: The expression of the multi-functional cellulase gene mfc in Coprinopsis cinerea is modulated using glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter fragments from Agaricus bisporus, Flammulina velutipes, and Lentinula edodes. In submerged fermentation, with banana peels as the growth substrate, highest activities of endo-β-1,4-glucanase (0.48 U/ml), total cellulase (0.26 U/ml) and endo-β-1,4-xylanase (38.10 U/ml) were noted in the culture liquid Cabm44, which uses a 275 bp gpd fragment from A. bisporus. The lignocellulolytic enzymatic activities were increased nearly twofold in comparison with the wild-type strain.

Keywords: Multi-functional cellulase; Coprinopsis cinerea; gpd promoters; Lignocellulose fermentation (search for similar items in EconPapers)
Date: 2011
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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:36:y:2011:i:12:p:3268-3272

DOI: 10.1016/j.renene.2011.04.019

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