 Formation of hydrogen-producing granules and microbial community analysis in a UASB reactorYan-Yan Ning, Shao-Feng Wang, Da-Wei Jin, Hideki Harada and Xian-Yang Shi Renewable Energy, 2013, vol. 53, issue C, 12-17 Abstract: The effect of substrate concentration on continuous H2 production from a sucrose-rich synthetic wastewater was investigated in this work. The maximum H2 production rate of 2.89 m3 H2/m3/d achieved at 7000 mg COD/L while the peak ethanol concentration of 2840 mg/L was observed at 5000 mg COD/L. The H2-producing granules with ethanol-type fermentation were successfully cultivated in an upflow anaerobic sludge blanket (UASB) reactor. Denaturing gradient gel electrophoresis analysis demonstrates that the substrate concentration had a considerable effect on the microbial community. After the mature H2-producing granules were formed, the microbial community structure in the UASB reactor tends to stabilize. The dominant bacterium in the mature H2-producing granules were composed of Janthinobacterium sp. WPCB148, Clostridium sp. HPB-4, Variovorax paradoxus strain SJ100, Variovorax sp. CN3b and Uncultured bacterium clone C2. Keywords: Hydrogen production; Upflow anaerobic sludge blanket (UASB) reactor; Granule; PCR-DGGE (search for similar items in EconPapers) Downloads: (external link) Related works: Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:53:y:2013:i:c:p:12-17 DOI: 10.1016/j.renene.2012.10.051 Access Statistics for this article Renewable Energy is currently edited by Soteris A. Kalogirou and Paul Christodoulides More articles in Renewable Energy from Elsevier |
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