Direct conversion of cassava starch into single cell oil by co-cultures of the oleaginous yeast Rhodosporidium toruloides and immobilized amylases-producing yeast Saccharomycopsis fibuligera

Qian Gen, Qi Wang and Zhen-Ming Chi

Renewable Energy, 2014, vol. 62, issue C, 522-526

Abstract: Some strains of Rhodosporidium toruloides can produce high concentrations of single cell oil. However, this oleaginous yeast does not produce amylases. Cells of the amylase-producing yeast Saccharomycopsis fibuligera A11-c were immobilized using polyvinyl alcohol. The immobilized yeast could produce 325 U/ml of amylase activity within 72 h of incubation. These amylases hydrolyzed cassava starch and the resulting product was converted into single cell oil by R. toruloides 21167. In a 2-l co-culture bioreactor, a single cell oil yield (64.9% w/w) from a cell mass of R. toruloides 21167 (20.1 g/l) were produced from cassava starch (6.0% w/v). Over 96% of the fatty acids produced were C16:0, C18:0, C18:1 and C18:2, useful for conversion into biodiesel.

Keywords: Oleaginous red yeast; Amylases-producing yeast; Lipid; Co-cultures; Cassava starch (search for similar items in EconPapers)
Date: 2014
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Citations: View citations in EconPapers (3)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:62:y:2014:i:c:p:522-526

DOI: 10.1016/j.renene.2013.08.016

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