Production, characterization of acetyl esterase from a rumen bacteria strain RB3, and application potential of the strain in biodegradation of crop residues

Jing Chen, Lixin Xu, Yue Wu, Jin Tong and Yuxiang Chen

Renewable Energy, 2014, vol. 68, issue C, 134-139

Abstract: Acetyl esterase was produced by a bacterial strain RB3 at a level of 0.59 U mL−1. The strain was isolated from beef cattle rumen fluid under anaerobic condition, and was identified as Escherichia coli. The peak activity of the enzyme appeared after 48 h of culturing under anaerobic condition. The optimal pH of the enzyme activity was 8.0, and the optimal temperature was 40 °C. The Km and Vmax values on p-nitrophenyl acetate were 0.84 mM and 0.13 mmol p-nitrophenol liberated min−1 mg of protein−1 respectively. The enzyme activity could be promoted by Zn2+, Ni2+, Fe2+, and K+, and inhibited by Cu2+, Fe3+, Mn2+, Mg2+, Ca2+, and Co2+. Biodegradation of rice stalk and maize stover by the strain RB3 and Pleurotus ostreatus was compared. The strain showed higher degradation rate for hemicellulose in the crop residues, while P. ostreatus showed higher degradation rate for cellulose. This indicated the potential industrial application of the strain RB3, particularly in utilizing renewable lignocellulose containing acetyl xylan for fermentation of products.

Keywords: Rumen bacteria; Acetyl esterase; Crop residues; Biodegradation (search for similar items in EconPapers)
Date: 2014
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0960148114000627
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:68:y:2014:i:c:p:134-139

DOI: 10.1016/j.renene.2014.01.033

Access Statistics for this article

Renewable Energy is currently edited by Soteris A. Kalogirou and Paul Christodoulides

More articles in Renewable Energy from Elsevier
Bibliographic data for series maintained by Catherine Liu ().