Isolation of a Clostridium acetobutylicum strain and characterization of its fermentation performance on agricultural wastes

Najeeb Kaid Nasser Al-Shorgani, Mohd Hafez Mohd Isa, Wan Mohtar Wan Yusoff, Mohd Sahaid Kalil and Aidil Abdul Hamid

Renewable Energy, 2016, vol. 86, issue C, 459-465

Abstract: A new solvent-producing Clostridium has been isolated from soil used in intensive rice cultivation. The 16S rRNA analysis of the isolate indicates that it is closely related to Clostridium acetobutylicum, with a sequence identity of 96%. The new isolate, named C. acetobutylicum YM1, produces biobutanol from multiple carbon sources, including glucose, fructose, xylose, arabinose, glycerol, lactose, cellobiose, mannitol, maltose, galactose, sucrose and mannose. This isolate can also utilize polysaccharides such as starch and carboxylmethyl cellulose (CMC) for the production of biobutanol. The ability of isolate YM1 to produce biobutanol from agro-industrial wastes was also evaluated for rice bran, de-oiled rice bran, palm oil mill effluent and palm kernel cake. The highest concentration of biobutanol (7.27 g/L) was obtained from the fermentation medium containing 2% (w/v) fructose, with a total acetone–butanol–ethanol (ABE) concentration of 10.23 g/L. The ability of isolate YM1 to produce biobutanol from various carbon sources and agro-wastes indicates the promise of the use of this isolate for the production of biobutanol, a renewable energy resource, from readily available renewable feedstocks.

Keywords: Isolation; Characterization; Biobutanol; Clostridium; Renewable energy (search for similar items in EconPapers)
Date: 2016
References: View complete reference list from CitEc
Citations: View citations in EconPapers (3)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:86:y:2016:i:c:p:459-465

DOI: 10.1016/j.renene.2015.08.051

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