Stepwise-Selected Bacillus amyloliquefaciens and B. subtilis Strains from Composted Aromatic Plant Waste Able to Control Soil-Borne Diseases

Massimo Zaccardelli, Roberto Sorrentino, Michele Caputo, Riccardo Scotti, Enrica De Falco and Catello Pane
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Massimo Zaccardelli: Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria, Centro di ricerca Orticoltura e Florovivaismo, Via Cavalleggeri 25, 84098 Pontecagnano Faiano (SA), Italy
Roberto Sorrentino: Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria, Centro di ricerca Orticoltura e Florovivaismo, Via Cavalleggeri 25, 84098 Pontecagnano Faiano (SA), Italy
Michele Caputo: Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria, Centro di ricerca Orticoltura e Florovivaismo, Via Cavalleggeri 25, 84098 Pontecagnano Faiano (SA), Italy
Riccardo Scotti: Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria, Centro di ricerca Orticoltura e Florovivaismo, Via Cavalleggeri 25, 84098 Pontecagnano Faiano (SA), Italy
Enrica De Falco: Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II 132, 84084 Fisciano (SA), Italy
Catello Pane: Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria, Centro di ricerca Orticoltura e Florovivaismo, Via Cavalleggeri 25, 84098 Pontecagnano Faiano (SA), Italy

Agriculture, 2020, vol. 10, issue 2, 1-13

Abstract: In the present study, 133 bacterial isolates from 11 composted aromatic plant wastes were selected for their ability to inhibit the mycelial growth of the soil-borne phytopathogenic fungi Sclerotinia minor and Rhizoctonia solani . Successively, a subset of 35 from them were further characterized for their ability to control, in vivo, rocket damping-off caused by the two fungi. Moreover, the isolates were characterized for morphology of the colonies, Gram reaction, siderophore production, P-solubilization and for the presence of antimicrobial lipopeptide genes in the genome. The screening for the in vitro antagonisms showed a mycelial growth reduction ranging between 31.7% and 56.1% for R. solani and 34.4% and 59.4% for S. minor . All the isolates were not able to produce siderophores and some of them were able to solubilize P. The isolates contained two or more of the five lipoproteins coding genes investigated in this study. The most promising isolates were identified at species level by 16S-rRNA partial gene sequence analysis and were grouped in two main clusters related to Bacillus subtilis and Bacillus amyloliquefaciens reference strains. Results indicated that Bacillus isolates from compost are good candidates for application in the biocontrol of cultivated plants.

Keywords: antagonists; biological control; compost; lipopedtide genes; sustainable agriculture (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2020
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