Pseudomonas fluorescens MZ05 Enhances Resistance against Setosphaeria turcica by Mediating Benzoxazinoid Metabolism in the Maize Inbred Line Anke35

Cheng Zhou, Zhongyou Ma, Xiaoming Lu, Lin Zhu and Congsheng Yan
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Cheng Zhou: Key Laboratory of Bio-organic Fertilizer Creation, Ministry of Agriculture, Anhui Science and Technology University, Bengbu 233100, China
Zhongyou Ma: Key Laboratory of Bio-organic Fertilizer Creation, Ministry of Agriculture, Anhui Science and Technology University, Bengbu 233100, China
Xiaoming Lu: Key Laboratory of Bio-organic Fertilizer Creation, Ministry of Agriculture, Anhui Science and Technology University, Bengbu 233100, China
Lin Zhu: Key Laboratory of Bio-organic Fertilizer Creation, Ministry of Agriculture, Anhui Science and Technology University, Bengbu 233100, China
Congsheng Yan: Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, China

Agriculture, 2020, vol. 10, issue 2, 1-14

Abstract: Beneficial rhizobacteria can inhibit foliar pathogen infection by activation of defense responses, yet it the mechanisms of rhizobacteria-induced disease resistance remain largely unknown. Here, inoculation of susceptible maize plants with Pseudomonas fluorescens MZ05 significantly reduced disease occurrence caused by the leaf pathogen Setosphaeria turcica . Gene expression profiles of MZ05-inoculated plants were investigated by RNA-sequencing analyses, showing that several differentially expressed genes were positively associated with the metabolic processes of benzoxazinoids. Accordantly, the inoculation with P. fluorescens MZ05 resulted in a significant increase in the levels of 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA) in the maize leaves. Furthermore, pre-inoculation with P. fluorescens MZ05 enhanced the transcription of two defense-related marked genes PAL and PR2a , as well as BX2 and GLU2 , which are involved in DIMBOA biosynthesis, in pathogen-infected leaves. Defense responses in the inoculated plants were also greatly stronger and quicker than that in non-inoculated plants after pathogen attacks. However, virus-mediated silencing of BX2 or GLU2 remarkably attenuated the MZ05-induced effects, as evidenced by more disease occurrence and lower transcription of PAL and PR2a . Collectively, these findings indicated that the MZ05-induced increases of DIMBOA levels participated in the mediation of priming, which was the key mechanism in the rhizobacteria-induced host resistance.

Keywords: beneficial rhizobacteria; induced disease resistance; benzoxazinoid; Setosphaeria turcica; DIMBOA (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2020
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