Overexpression of GmRIQ2-like ( Glyma.04G174400 ) Enhances the Tolerance of Strong Light Stress and Reduces Photoinhibition in Soybean ( Glycine max (L.) Merr.)

Jing Deng, Dongmei Li, Huayi Yin, Li Ma, Jiukun Zhang and Binbin Zhang
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Jing Deng: Key Laboratory of Soybean Biology in Chinese Education Ministry (Northeastern Key Laboratory of Soybean Biology and Genetics &Breeding in Chinese Ministry of Agriculture), Northeast Agricultural University, Harbin 150030, China
Dongmei Li: Key Laboratory of Soybean Biology in Chinese Education Ministry (Northeastern Key Laboratory of Soybean Biology and Genetics &Breeding in Chinese Ministry of Agriculture), Northeast Agricultural University, Harbin 150030, China
Huayi Yin: Key Laboratory of Soybean Biology in Chinese Education Ministry (Northeastern Key Laboratory of Soybean Biology and Genetics &Breeding in Chinese Ministry of Agriculture), Northeast Agricultural University, Harbin 150030, China
Li Ma: Key Laboratory of Soybean Biology in Chinese Education Ministry (Northeastern Key Laboratory of Soybean Biology and Genetics &Breeding in Chinese Ministry of Agriculture), Northeast Agricultural University, Harbin 150030, China
Jiukun Zhang: Key Laboratory of Soybean Biology in Chinese Education Ministry (Northeastern Key Laboratory of Soybean Biology and Genetics &Breeding in Chinese Ministry of Agriculture), Northeast Agricultural University, Harbin 150030, China
Binbin Zhang: Key Laboratory of Soybean Biology in Chinese Education Ministry (Northeastern Key Laboratory of Soybean Biology and Genetics &Breeding in Chinese Ministry of Agriculture), Northeast Agricultural University, Harbin 150030, China

Agriculture, 2020, vol. 10, issue 5, 1-20

Abstract: Soybean ( Glycine max (L.) Merr.) is an important crop that serves as a source of edible oil and protein. However, little is known about its molecular mechanism of adaptation to extreme environmental conditions. Based on the Arabidopsis thaliana sequence database and Phytozome, a soybean gene that had a highly similar sequence to the reduced induction of the non-photochemical quenching2 ( AtRIQ2 ) gene, GmRIQ2-like (accession NO.: Glyma.04G174400 ), was identified in this study. The gene structure analysis revealed that GmRIQ2-like encoded a transmembrane protein. Elements of the promoter analysis indicated that GmRIQ2-like participated in the photosynthesis and abiotic stress pathways. The subcellular localization results revealed that the protein encoded by GmRIQ2-like was located in chloroplasts. The quantitative real-time (qRT)-PCR results revealed that GmRIQ2-like- overexpression (OE) and -knock-out (KO) transgenic soybean seedlings were cultivated successfully. The relative chlorophyll (Chl) and zeaxanthin contents and Chl fluorescence kinetic parameters demonstrated that GmRIQ2-like dissipated excess light energy by enhancing the non-photochemical quenching (NPQ) and reduced plant photoinhibition. These results suggested that GmRIQ2-like was induced in response to strong light and depressed Chl production involved in soybean stress tolerance. These findings indicate that the transgenic seedlings of GmRIQ2-like could be used to enhance strong light stress tolerance and protect soybean plants from photoinhibition damage. This study will serve as a reference for studying crop photoprotection regulation mechanisms and benefits the research and development of new cultivars.

Keywords: soybean ( Glycine max (L.) Merr.); NPQ; photoinhibition; bioinformatics; subcellular localization; qRT-PCR (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2020
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