CsICE1 Functions in Cold Tolerance by Regulating Polyamine levels May through Interacting with Arginine Decarboxylase in the Tea Tree

Xujun Zhu, Xue Zhao, Taiyu Ren, Yuanchun Ma, Yuhua Wang and Wanping Fang
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Xujun Zhu: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing 210095, Jiangsu, China
Xue Zhao: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing 210095, Jiangsu, China
Taiyu Ren: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing 210095, Jiangsu, China
Yuanchun Ma: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing 210095, Jiangsu, China
Yuhua Wang: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing 210095, Jiangsu, China
Wanping Fang: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing 210095, Jiangsu, China

Agriculture, 2020, vol. 10, issue 6, 1-11

Abstract: Background: The identification of C-repeat binding factor (CBF), and the characterization as an inducer of CBF Expression 1 (ICE1), and a major activator for C-repeat binding factor, were important breakthroughs in the cold signaling network. Methods: In the present study, the full length cDNA of ICE1 was isolated from the tea tree ( Camellia sinensis ). CsICE1 protein was located in the cell nucleus as revealed by subcellular localization analysis. To investigate the biological functions of CsICE1 , a transgenic line fused with the CsICE1 gene in Arabidopsis thaliana (arabidopsis) was generated by the floral dip method. Results: The CsICE1 was expressed differentially in various tea tree tissues, mostly in buds and leaves, and the transcript level of CsICE1 was increased after 1 h and peaked at 2 h under cold treatment. Transcription activity assay indicated that the spermine synthase ( SPMS ) and arginine decarboxylase ( ADC ) genes were possible targets of CsICE1. In addition, the values of net photosynthetic rate, transpiration rate, stomatal conductance in transgenic lines declined by less extent than wild-type plants under low temperatures. Furthermore, transcript levels of ADC genes in the transgenic lines had no apparent alteration under normal growth conditions but substantially increased under cold conditions, consistent of changes in free polyamine levels. Taken together, these results demonstrated that CsICE1 plays a positive role in cold tolerance, which may be due to the modulation of polyamine levels through interacting with CsADC .

Keywords: biological function; Camellia sinensis; expression analysis; gene cloning; transgenic plant; arginine decarboxylase (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2020
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