Identification of a Novel QTL for Chlorate Resistance in Rice ( Oryza sativa L.)

Nkulu Rolly Kabange, So-Yeon Park, Dongjin Shin, So-Myeong Lee, Su-Min Jo, Youngho Kwon, Jin-Kyung Cha, You-Chun Song, Jong-Min Ko and Jong-Hee Lee
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Nkulu Rolly Kabange: Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
So-Yeon Park: Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
Dongjin Shin: Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
So-Myeong Lee: Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
Su-Min Jo: Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
Youngho Kwon: Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
Jin-Kyung Cha: Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
You-Chun Song: Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
Jong-Min Ko: Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
Jong-Hee Lee: Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea

Agriculture, 2020, vol. 10, issue 8, 1-16

Abstract: Chlorate resistance analysis is an effective approach commonly used to distinguish the genetic variation between Oryza sativa L. ssp. indica and japonica, and predict the nitrogen use efficiency (NUE). This study aimed at investigating the response of a doubled haploid (DH) population derived from anther culture of 93-11 × Milyang352 exposed to 0.1% potassium chlorate (KClO 3 ) at the seedling stage. The results revealed that the parental rice lines 93-11 ( indica ) and Milyang352 ( japonica ) showed distinctive phenotypic responses. The parental line 93-11 scored highly sensitive (0% survival) and Milyang352 scored resistant (66.7% survival) 7 days after treatment. The DH lines reflected the differential phenotypic response observed in parental lines. Interestingly, we identified a novel quantitative trait locus (QTL) for chlorate resistance on chromosome 3 (qCHR-3 , 136 cM, logarithm of the odds—LOD: 4.1) using Kompetitive Allele-Specific PCR (KASP) markers. The additive effect (−11.97) and phenotypic variation explained (PVE; 14.9%) indicated that the allele from Milyang352 explained the observed phenotypic variation. In addition, shoot growth showed a significant difference between parental lines, but not root growth. Moreover, in silico analysis identified candidate genes with diverse and interesting molecular and physiological functions. Therefore, this study suggested that the QTL qCHR-3 harbors promising candidate genes that could play a role in the regulation of nitrogen metabolism in rice.

Keywords: quantitative trait locus; chlorate resistance; nitrogen use efficiency; doubled haploid; rice (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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