Inorganic Phosphate Solubilization by a Novel Isolated Bacterial Strain Enterobacter sp. ITCB-09 and Its Application Potential as Biofertilizer

Gustavo Enrique Mendoza-Arroyo, Manuel Jesús Chan-Bacab, Ruth Noemi Aguila-Ramírez, Benjamín Otto Ortega-Morales, René Efraín Canché Solís, Antonio O. Chab-Ruiz, Katia I. Cob-Rivera, Benito Dzib-Castillo, Rodrigo Enrique Tun-Che and Juan Carlos Camacho-Chab
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Gustavo Enrique Mendoza-Arroyo: Laboratory of Microbiology, National Technological of Mexico, Campus Chiná, Campeche 24520, Campeche, Mexico
Manuel Jesús Chan-Bacab: Department of Environmental Microbiology and Biotechnology, Autonomous University of Campeche, Campeche 24039, Campeche, Mexico
Ruth Noemi Aguila-Ramírez: Microbiology and Molecular Biology Laboratory, National Polytechnic Institute (IPN), Interdisciplinary Center of Marine Sciences (CICIMAR), La Paz 23096, Baja California Sur, Mexico
Benjamín Otto Ortega-Morales: Department of Environmental Microbiology and Biotechnology, Autonomous University of Campeche, Campeche 24039, Campeche, Mexico
René Efraín Canché Solís: Department of Chemical and Biochemical Engineering, Campus Campeche, National Technological of Mexico, Lerma, Campeche 24500, Campeche, Mexico
Antonio O. Chab-Ruiz: Laboratory of Microbiology, National Technological of Mexico, Campus Chiná, Campeche 24520, Campeche, Mexico
Katia I. Cob-Rivera: Laboratory of Microbiology, National Technological of Mexico, Campus Chiná, Campeche 24520, Campeche, Mexico
Benito Dzib-Castillo: Postgraduate Unit, National Technological of Mexico, Campus Chiná. Campeche 24520, Campeche, Mexico
Rodrigo Enrique Tun-Che: Department of Environmental Microbiology and Biotechnology, Autonomous University of Campeche, Campeche 24039, Campeche, Mexico
Juan Carlos Camacho-Chab: Department of Environmental Microbiology and Biotechnology, Autonomous University of Campeche, Campeche 24039, Campeche, Mexico

Agriculture, 2020, vol. 10, issue 9, 1-15

Abstract: The excessive use of fertilizers in agriculture is mainly due to the recognized plant requirements for soluble phosphorus. This problem has limited the implementation of sustainable agriculture. A viable alternative is to use phosphate solubilizing soil microorganisms. This work aimed to isolate inorganic phosphorus-solubilizing bacteria from the soils of agroecosystems, to select and identify, based on sequencing and phylogenetic analysis of the 16S rRNA gene, the bacterium with the highest capacity for in vitro solubilization of inorganic phosphate. Additionally, we aimed to determine its primary phosphate solubilizing mechanisms and to evaluate its effect on Habanero pepper seedlings growth. A total of 21 bacterial strains were isolated by their activity on Pikovskaya agar. Of these, strain ITCB-09 exhibited the highest ability to solubilize inorganic phosphate (865.98 µg/mL) through the production of organic acids. This strain produced extracellular polymeric substances and siderophores that have ecological implications for phosphate solubilization. 16S rRNA gene sequence analysis revealed that strain ITCB-09 belongs to the genus Enterobacter . Enterobacter sp. ITCB-09, especially when immobilized in beads, had a positive effect on Capsicum chinense Jacq. seedling growth, indicating its potential as a biofertilizer.

Keywords: biofertilizers; Enterobacter sp.; phosphate solubilization; siderophores; extracellular polymeric substances (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2020
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