Bacillus velezensis T149-19 and Bacillus safensis T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato

Jackeline Rossetti Mateus, Isabella Dal’Rio, Diogo Jurelevicius, Fabio Faria da Mota, Joana Montezano Marques, Rommel Thiago Juca Ramos, Artur Luiz da Costa da Silva, Paulo Roberto Gagliardi and Lucy Seldin
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Jackeline Rossetti Mateus: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil
Isabella Dal’Rio: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil
Diogo Jurelevicius: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil
Fabio Faria da Mota: Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Rio de Janeiro CEP 21040-360, Brazil
Joana Montezano Marques: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil
Rommel Thiago Juca Ramos: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém CEP 66075-110, Brazil
Artur Luiz da Costa da Silva: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém CEP 66075-110, Brazil
Paulo Roberto Gagliardi: Departamento de Engenharia Agronômica do Campus de São Cristóvão, Universidade Federal do Sergipe, Sergipe CEP 49100-000, Brazil
Lucy Seldin: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil

Agriculture, 2021, vol. 11, issue 11, 1-14

Abstract: Sweet potato ( Ipomoea batatas ) tuberous roots are used for human consumption, animal feed, and many industrial products. However, the crop is susceptible to various pests and diseases, including foot rot disease caused by the phytopathogenic fungus Plenodomus destruens . Biological control of plant pathogens by Bacillus species is widely disseminated in agrosystems, but specific biological control agents against the foot rot disease-causing fungus are not yet available. Our previous studies showed that two Bacillus strains isolated from sweet potato roots— B. safensis T052-76 and B. velezensis T149-19—were able to inhibit P. destruens in vitro, but data from in vivo experiments using simultaneously the fungus and the bacteria were missing. In this study, both strains were shown to protect the plant from the disease and to mitigate the symptoms of foot rot disease in pot experiments. Total fungal community quantification using real-time PCR showed a significant decrease in the number of copies of the ITS gene when the bacteria were inoculated, compared to the control (with the fungus only). To determine the genes encoding antimicrobial substances likely to inhibit the fungus, their genomes were sequenced and annotated. Genes coding for mycosubtilin, bacillaene, macrolactin, bacillibactin, bacilysin, plantazolicin, plipastatin, dificidine, fengycin and surfactin were found in B. velezensis T149-19, while those coding for bacylisin, lichenysin, bacillibactin, fengycin and surfactin were found in B. safensis T052-76. Altogether, the data presented here contribute to advancing the knowledge for the use of these Bacillus strains as biocontrol products in sweet potato.

Keywords: Bacillus velezensis; Bacillus safensis; Plenodomus destruens; foot rot disease; biocontrol; sweet potato (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2021
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