Isolation and Identification Rust Pathogens and the Study of Antioxidant Enzyme Activity and Gene Expression under Rust Infection in Zoysia japonica

Di Zhang, Hanguo Zeng, Liyuan Zhao, Jiaming Yue, Xiao Qi and Manli Li
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Di Zhang: College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
Hanguo Zeng: College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
Liyuan Zhao: College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
Jiaming Yue: College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
Xiao Qi: National Animal Husbandary Service, Ministry of Agriculture and Rural Affairs, Beijing 100025, China
Manli Li: College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China

Agriculture, 2021, vol. 11, issue 12, 1-14

Abstract: The goal of this study was to identify the zoysiagrass rust pathogens and to analyze the differences in rust-resistant and rust-susceptible Zoysia japonica germplasm upon inoculation. Based on the assessment of spore morphology and 18S ribosomal DNA (rDNA) molecular identification, the zoysiagrass rust pathogen was identified as Puccinia zoysiae Diet. The development of mycelium, the rate of spreading, and the timing of spore production were more delayed in the rust-resistant (RR) genotype than the rust-susceptible (RS) genotype. After inoculation, the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) initially decreased, then increased in both the RR and RS genotypes, but the increased enzyme activities were faster in the RR than in the RS genotype. Rust resistance was positively correlated with antioxidant enzyme activity. The observed changes in CAT, POD and APX activity corresponded to their gene expression levels. The results of this study may be utilized in accurately evaluating the damage of rust disease and rust-resistance in zoysiagrass germplasm aimed at breeding the rust-resistant zoysiagrass varieties and improving disease management of zoysiagrass turf.

Keywords: zoysiagrass rust; 18S rDNA; antioxidant enzyme; gene expression; scanning electron microscope (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2021
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