Biosystematic Study on Some Egyptian Species of Astragalus L. (Fabaceae)

Monier M. Abd El-Ghani, Ashraf S. A. El-Sayed, Ahmed Moubarak, Rabab Rashad, Hala Nosier and Adel Khattab
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Monier M. Abd El-Ghani: Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt
Ashraf S. A. El-Sayed: Department of Botany and Microbiology, Faculty of Science, Zagazig University, Al Sharqiya Governorate 44519, Egypt
Ahmed Moubarak: Department of Botany and Microbiology, Faculty of Science, Benha University, Benha 13518, Egypt
Rabab Rashad: Department of Botany and Microbiology, Faculty of Science, Benha University, Benha 13518, Egypt
Hala Nosier: Department of Botany, Faculty of Science, Ain Shams University, Cairo 11566, Egypt
Adel Khattab: Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt

Agriculture, 2021, vol. 11, issue 2, 1-16

Abstract: Astragalus L. is one of the largest angiosperm complex genera that belongs to the family Fabaceae, subfamily Papilionoideae or Faboideae under the subtribe Astragalinae of the tribe Galegeae. The current study includes the whole plant morphology, DNA barcode (ITS2), and molecular marker (SCoT). Ten taxa representing four species of Astragalus were collected from different localities in Egypt during the period from February 2018 to May 2019. Morphologically, identification and classification of collected Astragalus plants occurred by utilizing the light microscope, regarding the taxonomic revisions of the reference collected Astragalus specimens in other Egyptian Herbaria. For molecular validation, ten SCoT primers were used in this study, producing a unique banding pattern to differentiate between ten samples of Astragalus taxa which generated 212 DNA fragments with an average of 12.2 bands per 10 Astragalus samples, with 8 to 37 fragments per primer. The 212 fragments amplified were distributed as 2 monomorphic bands, 27 polymorphic without unique bands, 183 unique bands (210 Polymorphic with unique bands), and ITS2 gene sequence was showed as the optimal barcode for identifying Astragalus L. using BLAST searched on NCBI database, and afterward, analyzing the chromatogram for ITS region, 10 samples have been identified as two samples representing A. hauarensis , four samples representing A. sieberi , three samples representing A. spinosus and one sample representing A. vogelii . Based on the ITS barcode, A. hauarensis RMG1, A. hauarensis RMG2, A. sieberi RMG1, A. sieberi RMG2, A. sieberi RMG3, A. sieberi RMG4, A. spinosus RMG1, A. spinosus RMG2, A. spinosus RMG3, A. vogelii RMG were deposited into GenBank with accession # MT367587.1, MT367591.1, MT367593.1, MT367585.1, MT367586.1, MT367588.1, MT160347.1, MT367590.1, MT367589.1, MT367592.1, respectively. These results indicated the efficiency of SCoT markers and ITS2 region in identifying and determining genetic relationships between Astragalus species.

Keywords: morphology; DNA extraction; SCoT polymorphism; ITS2 region; phylogeny (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2021
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