Halotolerant Endophytic Bacterium Serratia rubidaea ED1 Enhances Phosphate Solubilization and Promotes Seed Germination

Ismail Mahdi, Mohamed Hafidi, Abdelmounaaim Allaoui and Latefa Biskri
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Ismail Mahdi: Medical Applications Interface Center (CIAM), Mohammed VI Polytechnic University (UM6P), Ben Guerir 43150, Morocco
Mohamed Hafidi: Laboratory of Microbial Biotechnologies, Agrosciences and Environment, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh 40000, Morocco
Abdelmounaaim Allaoui: Laboratory of Molecular Microbiology, Coalition Center, for Innovation, and Prevention of Epidemics in Morocco (CIPEM), Mohammed VI Polytechnic University (UM6P), Ben Guerir 43150, Morocco
Latefa Biskri: Laboratory of Molecular Microbiology, Coalition Center, for Innovation, and Prevention of Epidemics in Morocco (CIPEM), Mohammed VI Polytechnic University (UM6P), Ben Guerir 43150, Morocco

Agriculture, 2021, vol. 11, issue 3, 1-22

Abstract: Quinoa is renowned for its nutritional value and ability to withstand harsh environmental conditions such as salinity. In the present work, we isolated 34 phosphate solubilizing endophytic bacteria associated with the roots of quinoa plants. Based on phosphate solubilization efficiency and biochemical characterization, we selected one isolate named ED1. Ribotyping using partial 16S RNA gene analysis revealed that the selected isolate shares 99.7% identity with Serratia rubidaea . Plant growth promoting (PGP) studies showed that the ED1 strain solubilized complexed forms of phosphate (Ca 3 (PO 4 ) 2 ). Zinc release from ZnO, Zn 3 (PO 4 ) 2 , or ZnCO 3 revealed the efficient ZnO solubilization by the ED1 strain. Except for proteases, the strain ED1 produced siderophores, cellulase, ammonia and exhibited oligonitrophilic features. Indole acetic acid (IAA) production was detected with and without the L-tryptophan precursor. Next, we demonstrated that the ED1 strain tolerated 1.5 M NaCl final concentration and exhibited intrinsic resistance to seven antibiotics frequently prescribed for medical use. Moreover, we found that ED1 strain withstood 2 mg/L of Cadmium and 1 mg/L of either Nickel or Copper. Furthermore, we observed that S. rubidaea ED1 stimulated quinoa seeds germination and seedlings growth under salt stress conditions. Lastly, we discuss the advantages versus disadvantages of applying the S. rubidaea ED1 strain as a beneficial agent for salty and/or heavy metals contaminated soils.

Keywords: endophyte; Chenopodium quinoa willd.; Serratia rubidaea; plant growth promotion; salt stress; antibiotics resistance; heavy metals tolerance (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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