Transcription Profile Analysis of Chlorophyll Biosynthesis in Leaves of Wild-Type and Chlorophyll b -Deficient Rice ( Oryza sativa L.)

Minh Khiem Nguyen, Tin-Han Shih, Szu-Hsien Lin, Jun-Wei Lin, Hoang Chinh Nguyen, Zhi-Wei Yang and Chi-Ming Yang
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Minh Khiem Nguyen: Biodiversity Program, Taiwan International Graduate Program, Biodiversity Research Center, Academia Sinica and National Taiwan Normal University, Taipei 115, Taiwan
Tin-Han Shih: Biodiversity Research Center, Academia Sinica, Taipei 115, Taiwan
Szu-Hsien Lin: Biodiversity Research Center, Academia Sinica, Taipei 115, Taiwan
Jun-Wei Lin: Biodiversity Research Center, Academia Sinica, Taipei 115, Taiwan
Hoang Chinh Nguyen: Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam
Zhi-Wei Yang: Taoyuan District Agricultural Research and Extension Station, Council of Agriculture, Taoyuan 327, Taiwan
Chi-Ming Yang: Biodiversity Program, Taiwan International Graduate Program, Biodiversity Research Center, Academia Sinica and National Taiwan Normal University, Taipei 115, Taiwan

Agriculture, 2021, vol. 11, issue 5, 1-17

Abstract: Photosynthesis is an essential biological process and a key approach for raising crop yield. However, photosynthesis in rice is not fully investigated. This study reported the photosynthetic properties and transcriptomic profiles of chlorophyll (Chl) b -deficient mutant ( ch11 ) and wild-type rice ( Oryza sativa L.). Chl b -deficient rice revealed irregular chloroplast development (indistinct membranes, loss of starch granules, thinner grana, and numerous plastoglobuli). Next-generation sequencing approach application revealed that the differential expressed genes were related to photosynthesis machinery, Chl-biosynthesis, and degradation pathway in ch11 . Two genes encoding PsbR (PSII core protein), FtsZ1 , and PetH genes, were found to be down-regulated. The expression of the FtsZ1 and PetH genes resulted in disrupted chloroplast cell division and electron flow, respectively, consequently reducing Chl accumulation and the photosynthetic capacity of Chl b -deficient rice. Furthermore, this study found the up-regulated expression of the GluRS gene, whereas the POR gene was down-regulated in the Chl biosynthesis and degradation pathways. The results obtained from RT-qPCR analyses were generally consistent with those of transcription analysis, with the exception of the finding that MgCH genes were up-regulated which enhance the important intermediate products in the Mg branch of Chl biosynthesis. These results indicate a reduction in the accumulation of both Chl a and Chl b . This study suggested that a decline in Chl accumulation is caused by irregular chloroplast formation and down-regulation of POR genes; and Chl b might be degraded via the pheophorbide b pathway, which requires further elucidation.

Keywords: Chl b -deficient mutant; photosynthesis; RNA-Seq transcriptome (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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